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Flow cytometry to assess oxidative stress in cats

May 08, 2007
icon-blog Winn Funded Research

Webb, C., C. Bedwell, et al. (2006). "Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes." Vet Immunol Immunopathol 112(3-4): 129-40.

Oxidative stress and abnormal glutathione metabolism is thought to play an important role in various diseases of cats. However, current assays for the reduced form of glutathione (GSH) are time-consuming and semi-quantitative and do not allow assessment of GSH concentrations in individual cell populations. Therefore, we developed a flow cytometric assay for rapid determination of intracellular GSH concentrations in feline blood leukocytes.
>> PubMed abstract
flow cytometry cat health cat cat care oxidative stress Winn feline feline health leukocytes

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