Leblanc, C.J., L.L. Head, and M.M. Fry, Comparison of aspiration and nonaspiration techniques for obtaining cytologic samples from the canine and feline spleen. Vet Clin Pathol, 2009. 38(2): p. 242-6.
The objective of the study was to compare the quality of splenic cytology specimens obtained using aspiration versus nonaspiration techniques. Along with 24 dogs, 7 client-owned cats were evaluated. Two clinical pathologists were used to blindly and independently score the samples for cellularity, amount of blood, and preservation of cellular morphology. The aspiration technique is performed by using a 22-25 G needle and a 6-20 mL syringe to apply suction and obtain negative pressure for cellular yield. In the nonaspiration technique, a 22-25 G needle is guided into the target organ and incompletely retracted and redirected several times in an attempt to shear and displace cells into the cylinder of the needle. The authors concluded the nonaspiration technique is recommended for obtaining more cellular, less hemodiluted cytologic specimens of the feline spleen. [VT]Related articles:Ballegeer, E.A., et al., Correlation of ultrasonographic appearance of lesions and cytologic and histologic diagnoses in splenic aspirates from dogs and cats: 32 cases (2002-2005). J Am Vet Med Assoc, 2007. 230(5): p. 690-6.